?AUTOLYSIS OF PROTEOLYTIC ENZYME IN METASTATIC BREAST CANCEROUS TISSUE

Authors

  • Rukhshan Khurshid Govt lslamia College, Railway Road, Lahore, Pakistan
  • Mohd Saleem Akhtar Department of Pharmacology, Fatima Jinnan Medical College, Lahore, Pakistan
  • Nasim Akhte Department of Biochemistry, Fatima Jinnali Medical College, Lahore, Pakistan
  • Mumtaz Begum Department of Biochemistry, Fatima Jinnali Medical College, Lahore, Pakistan

Keywords:

Proteases, Chromatography, Breast cancer, Autolysis.

Abstract

Studies on autolysis of proteolytic enzyme like cathepsin revealed that the purified enzyme was autolysed by
metastasisorduetoabsenceofstabilizer.CrudetissuewasextractedwithsodiumchloridebuffercontainingEDTAand
Triton x-I00.Extract was analyzedfortotal proteinandproteaseactivity.Protein/peptide was analyzed/purified using
SDS polyacrylamide gelelectrophoresis, ionexchange chromatography and reversed phase HPLC. Results indicated
the autolysis during purification of enzyme. It is concluded that during and after purification suitable stabilizer of
enzyme should be added. In vitro experiment indicated that in vivo (cancerous tissue), the imbalance of specific
stabilizer/inhibitors of proteasecause autolysisof enzyme and theresulting peptides cause the damaging effects.

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Published

2001-06-18

How to Cite

Khurshid, R., Akhtar, M. S., Akhte, N., & Begum, M. (2001). ?AUTOLYSIS OF PROTEOLYTIC ENZYME IN METASTATIC BREAST CANCEROUS TISSUE. Biological Sciences - PJSIR, 44(3), 159–162. Retrieved from http://biostaging.pjsir.org/index.php/biological-sciences/article/view/1903

Issue

Vol. 44 No. 3 (2001): Pakistan Journal of Scientific and Industrial Research

Section

Articles