?MICROPROPAGATION POTENTIAL OF POLIANTHES TUBEROSA L BULBS, SCALES AND LEAVES

Abstract

To enhance the rate of multiplication of Polianthes tuberosa, in vitro techniques were applied. The micropropagation
potential of bulb and leaf tissues was explored. Twin scale and chip cxplants from .the lower halves and single scale
explants from the upper halves of the bulbs were cultured on MS medium supplemented with diIferent concentrations
of naphthalene acetic acid (NAA) and benzyl aminopurinc (BAP). bulblet induction was pronounced on both twin
scales and chips. Bulblet formation and gain in size was greater inthe case of chips than in twin scales, indicating an
influence of the size of the explant. Bulblet induction on single scale explants oftuberose bulbs was also observed. Leaf
blade explants formed callus on MS medium supplemented with defined hormonal concentrations of BAP, *AA and
Kin. Psuedoernbryogenic bodies regenerated from the calli.